Designs guide sequences for CRISPR/Cas9 genome editing and provides information on sequence features pertinent to guide efficiency. Sequence features include annotated off-target predictions in a user-selected genome and a predicted efficiency score based on the model described in Doench et al. (2016) <doi:10.1038/nbt.3437>. Users are able to import additional genomes and genome annotation files to use when searching and annotating off-target hits. All guide sequences and off-target data can be generated through the 'R' console with sgRNA_Design() or through 'crispRdesignR's' user interface with crispRdesignRUI(). CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and the associated protein Cas9 refer to a technique used in genome editing.
| Version: | 1.1.5 |
| Depends: | R (≥ 2.10) |
| Imports: | Biostrings, gbm, GenomicRanges, BiocGenerics, IRanges, GenomeInfoDb, S4Vectors, rtracklayer, stringr, vtreat, shiny, DT |
| Suggests: | BSgenome.Scerevisiae.UCSC.sacCer2 |
| Published: | 2020-05-26 |
| Author: | Dylan Beeber [aut, cre], Frederic Chain [aut] |
| Maintainer: | Dylan Beeber <dylan.beeber at gmail.com> |
| License: | GPL-3 |
| URL: | <https://github.com/dylanbeeber/crispRdesignR> |
| NeedsCompilation: | no |
| CRAN checks: | crispRdesignR results |
| Reference manual: | crispRdesignR.pdf |
| Package source: | crispRdesignR_1.1.5.tar.gz |
| Windows binaries: | r-devel: crispRdesignR_1.1.5.zip, r-release: crispRdesignR_1.1.5.zip, r-oldrel: crispRdesignR_1.1.5.zip |
| macOS binaries: | r-release: crispRdesignR_1.1.5.tgz, r-oldrel: crispRdesignR_1.1.5.tgz |
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