hagis

Project Status: Active – The project has reached a stable, usable state and is being actively developed. CRAN_Status_Badge Travis Build Status Appveyor Build Status codecov DOI

Introduction

The goal of hagis is to provide analysis tools for plant pathogens with gene-for-gene interactions in the R programming language that the original Habgood-Gilmour Spreadsheet, HaGiS, (Herrmann, Löwer and Schachtel) provided.

Overview

hagis was initially created for Phytophthora sojae surveys by Austin McCoy and Zachary Noel at Michigan State University in the US where the disease has been managed primarily via deployment of resistance genes (Rps genes, resistance to P. sojae) in commercial soybean cultivars and by the application of fungicide seed treatments. However, repeated use of resistance genes can cause populations to adapt over time rendering these resistance genes ineffective. To determine current effectiveness of resistance genes for managing P. sojae, state-wide surveys (in the US) are conducted to determine the pathotype (previously referred to as “race”) structure within sampled population of P. sojae.

However, the package is not only useful for P. sojae work. It was built to be useful for other plant pathogen gene-for-gene interaction systems, e.g. rusts or canola blackleg, Leptosphaeria maculans. The goal of this package is to provide all the necessary analyses needed when conducting a pathotype surveys, including: distribution of susceptibilities (effective and non-effective resistance genes), distribution of pathotype complexities with statistics, pathotype frequency distribution, as well as diversity indices for pathotypes in an efficient and reproducible manner.

New users are encouraged to visit the documentation, https://openplantpathology.github.io/hagis/articles/hagis.html, for detailed information on how to use hagis along with working examples using a built-in data set.

Quick Start Install

Stable Version

A stable version of hagis is available from CRAN.

install.packages("hagis")

Development Version

A development version is available from from GitHub. If you wish to install the development version that may have new features or bug fixes before the CRAN version does (but also may not work properly), please install the remotes package, available from CRAN. We strive to keep the master branch on GitHub functional and working properly.

if (!require("remotes")) {
  install.packages("remotes", repos = "http://cran.rstudio.com/")
  library("remotes")
}

install_github("openplantpathology/hagis", build_vignettes = TRUE)

Meta

Citation

When you use hagis, please cite by using:

citation("hagis")
#> 
#> Thank you for citing `hagis`.  To cite hagis in publications, please
#> use both the MPMI citation and the package version citation for
#> reproducibility purposes.
#> 
#>   Austin Glenn McCoy, Zachary A. Noel, Adam H. Sparks and Martin I.
#>   Chilvers (2019). `hagis', an R Package Resource for Pathotype
#>   Analysis of Phytophthora sojae Populations Causing Stem and Root Rot
#>   of Soybean. Molecular Plant-Microbe Interactions 32.12 (Nov 2019) p.
#>   1574-1576. DOI: 10.1094/MPMI-07-19-0180-A
#> 
#> McCoy AG, Noel ZA, Sparks AH, Chilvers MI (????). _hagis: Analysis of
#> Plant Pathogen Pathotype Complexities, Distributions and Diversity_.
#> doi: 10.5281/zenodo.2619820 (URL:
#> https://doi.org/10.5281/zenodo.2619820), R package version 3.0.0.9000,
#> <URL: https://openplantpathology.github.io/hagis/>.
#> 
#> To see these entries in BibTeX format, use 'print(<citation>,
#> bibtex=TRUE)', 'toBibtex(.)', or set
#> 'options(citation.bibtex.max=999)'.

Please note that the hagis project is released with a Contributor Code of Conduct. By contributing to this project, you agree to abide by its terms.

References

Herrmann, Löwer, and Schachtel, (1999), A new tool for entry and analysis of virulence data for plant pathogens. Plant Pathology, 48: 154-158. DOI: 10.1046/j.1365-3059.1999.00325.x